bsm-52989R [Primary Antibody]
PRKAR2B (5G1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PRKAR2B

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5577

Swiss Prot: P31323

Source: N terminal human PRKAR2B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

PRKAR2B is a regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 46


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of N2A cells with PRKAR2B (5G1) Monoclonal Antibody (bsm-52989R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


IF(ICC) staining with PRKAR2B (5G1) Monoclonal Antibody (bsm-52989R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PRKAR2B (5G1) Monoclonal Antibody (bsm-52989R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PRKAR2B (5G1) Monoclonal Antibody (bsm-52989R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PRKAR2B (5G1) Monoclonal Antibody (bsm-52989R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.