bsm-53000M [Primary Antibody]
RbAp 46/48 Monoclonal Antibody
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Host: Mouse

Target Protein: RbAp 46/48

Clonality: Monoclonal

Isotype: IgG2b

Entrez Gene: 5928

Swiss Prot: Q09028

Source: Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastoma-binding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46

Purification: Ascites fluid from mouse containing 0.05% azide

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

Size: 1mg

Concentration: Not Determined

Applications: WB(1:300-5000)
IF(ICC)(1:50-200)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot was performed using nuclear extracts from HeLa cells (20 μg) and RbAp 46/48 Monoclonal Antibody (bsm-53000M) diluted 1:1,000 in TBS-Tween containing 5% skim milk, followed by secondary antibody incubation and exposure.