bsm-53025M [Primary Antibody]
ORC2 (5C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: ORC2

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 4999

Swiss Prot: Q13416

Source: Monoclonal antibody raised in mouse against human ORC2, using two recombinant proteins containing the N-terminal and C-terminal part of the protein, respectively

Purification: Protein G purified monoclonal antibody in PBS containing 0.05% azide.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3.

Size: 50ug

Concentration: 0.6ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

RIPA cell lysates from HeLa cells (30 μg) were analysed by Western blot using ORC2 (5C3) Monoclonal Antibody (bsm-53025M) diluted 1:500 in PBS-Tween containing 10% milk. The position of the protein of interest is indicated on the right (expected size: 66 kDa); the marker (in kDa) is shown on the left.