bsm-54010R [Primary Antibody]
GCLC (1A5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GCLC

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2729

Swiss Prot: P48506

Source: Human GCLC aa 1-100

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. 

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 73


Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

A431 cell lysate probed with GCLC (1A5) Monoclonal Antibody (bsm-54010R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of Jurkay cells with GCLC (1A5) Monoclonal Antibody (bsm-54010R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded human fetal kidney tissue incubated with GCLC (1A5) Monoclonal Antibody (bsm-54010R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human fetal liver tissue incubated with GCLC (1A5) Monoclonal Antibody (bsm-54010R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded rat kidney tissue incubated with GCLC (1A5) Monoclonal Antibody (bsm-54010R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.