DATASHEET
Host:
Rabbit
Target Protein:
GATA1
Immunogen Range:
1-250/413
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
2623
Swiss Prot:
P15976
Source:
Human GATA1 between 1-200 amino acids
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells.
VALIDATION IMAGES
K562 cell lysate probed with GATA1 (8C3) Monoclonal Antibody (bsm-54013R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded human breast cancer tissue incubated with GATA1 (8C3) Monoclonal Antibody (bsm-54013R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded human liver tissue incubated with GATA1 (8C3) Monoclonal Antibody (bsm-54013R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.