bsm-54015R
[Primary Antibody]
CD163 (8G1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD163
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: Q86VB7
Source: Recombinant human CD163 between 1 to 250 amino acids
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 150
Cross Reactive Species:
Human
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhen Han. et al. Blockade of the Immune Checkpoint CD47 by TTI-621 Potentiates the Response to Anti-PD-L1 in Cutaneous T Cell Lymphoma. J INVEST DERMATOL. 2023 MaRead more>>
- Zhao Juan. et al. IRAK4 inhibition: an effective strategy for immunomodulating peri-implant osseointegration via reciprocally-shifted polarization in the monocyte-macrophage lineage cells. BMC ORAL HEALTH. 2023 Dec;23(1):1-17Read more>>
- Xin-Yue Yang. et al. Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice. J ETHNOPHARMACOL. 2024 Jan;318:117078Read more>>
- Cui Lu. et al. Combining decellularized adipose tissue with decellularized adventitia extravascular matrix or small intestinal submucosa matrix for the construction of vascularized tissue-engineered adipose. ACTA BIOMATER. 2023 SepRead more>>
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Mouse liver, 2: Rat spleen Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 121 kDa Observed MW: 150 kDa
Human thymus tissue lysate probed with CD163 (8G1) Monoclonal Antibody (bsm-54015R)at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human heart tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: THP-1 (Negative control), 2: Human liver Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 121 kDa Observed MW: 150 kDa
Tissue: Human liver Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54015R
Tissue: Human placenta Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54015R
Tissue: Mouse liver Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54015R
Tissue: Rat liver Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54015R
Paraformaldehyde-fixed and paraffin-embedded human liver tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded human placenta tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Flow cytometric analysis of thp-1 cells with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: THP-1 (Negative control), 2: Human liver Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 121 kDa Observed MW: 150 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Mouse liver, 2: Rat spleen Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 121 kDa Observed MW: 150 kDa
Tissue: Human liver Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54015R
Tissue: Human placenta Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54015R
Tissue: Mouse liver Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54015R
Tissue: Rat liver Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54015R
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD163 (8G1)) Monoclonal Antibody, Unconjugated (bsm-54015R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD163 (8G1)) Monoclonal Antibody, Unconjugated (bsm-54015R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD163 (8G1)) Monoclonal Antibody, Unconjugated (bsm-54015R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD163 (8G1)) Monoclonal Antibody, Unconjugated (bsm-54015R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Spleen tissue lysates; Lane 2: Rat Lung tissue lysates probed with CD163 Monoclonal Antibody, Unconjugated (bsm-54015R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.