bsm-54015R-HRP [Conjugated Primary Antibody]
CD163 Recombinant Antibody, HRP Conjugated
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD163

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9332

Swiss Prot: Q86VB7

Source: KLH conjugated synthetic peptide derived from human CD163

Purification: purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
IHC-P(1:200-800)
IHC-F(1:200-800)
IF(1:200-800)

Predicted Molecular Weight: 130 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhen Han. et al. Blockade of the Immune Checkpoint CD47 by TTI-621 Potentiates the Response to Anti-PD-L1 in Cutaneous T Cell Lymphoma. J INVEST DERMATOL. 2023 MaRead more>>
  • Zhao Juan. et al. IRAK4 inhibition: an effective strategy for immunomodulating peri-implant osseointegration via reciprocally-shifted polarization in the monocyte-macrophage lineage cells. BMC ORAL HEALTH. 2023 Dec;23(1):1-17Read more>>
  • Xin-Yue Yang. et al. Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice. J ETHNOPHARMACOL. 2024 Jan;318:117078Read more>>
  • Cui Lu. et al. Combining decellularized adipose tissue with decellularized adventitia extravascular matrix or small intestinal submucosa matrix for the construction of vascularized tissue-engineered adipose. ACTA BIOMATER. 2023 SepRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD163 Monoclonal Antibody (bsm-54015R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD163 Monoclonal Antibody (bsm-54015R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD163 Monoclonal Antibody (bsm-54015R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD163 Monoclonal Antibody (bsm-54015R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human serous fluid carcinoma of ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD163 Monoclonal Antibody (bsm-54015R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD163 Monoclonal Antibody (bsm-54015R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD163 Monoclonal Antibody (bsm-54015R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.