bsm-54021R [Primary Antibody]
Annexin A2 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Annexin A2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 302

Swiss Prot: P07355

Source: Human Annexin A2 aa 1-200

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain which binds phospholipids in a Ca2+-dependent manner and a unique amino-terminal region which may confer binding specificity. The interaction between these proteins and biological membranes has led to the hypothesis that they are involved in cellular trafficking processes such as endocytosis, exocytosis and cellular adhesion. Annexin II, also called p36, exists as a monomer or as a heterotetramer, complexed with the S-100-related protein p11. This complex is termed calpactin I. In the tetrameric form, Annexin II is an efficient substrate of PKC family and Src pp60.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 38


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

IF(ICC) staining with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of K562 cells with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human U251 cell lysates probed with Annexin A2 Monoclonal Antibody, Unconjugated (bsm-54021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.