bsm-54024R [Primary Antibody]
hnRNP A1 (4H7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: hnRNP A1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3178

Swiss Prot: P09651

Source: Human hnRNP A1 aa 300 to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. The A/B subfamily of hnRNPs include A1, A2/B1, A3 and A0, and in Xenopus, hnRNP A1, A2 and A3 are ubiquitously expressed throughout development as well as in adult tissues. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The hnRNP A0 gene is distinct from the other A/B family members, and it encodes a low-abundance protein, which is implicated in mRNA stability.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HepG2 Cells; Lane 2: PC-12 Cells; Probed with hnRNP A1 (4H7) Monoclonal Antibody (bsm-54024R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with hnRNP A1 (4H7) Monoclonal Antibody (bsm-54024R) at 1:100 in MCF-7cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


F(ICC) staining with hnRNP A1 (4H7) Monoclonal Antibody (bsm-54024R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with hnRNP A1 (4H7) Monoclonal Antibody (bsm-54024R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with hnRNP A1 (4H7) Monoclonal Antibody (bsm-54024R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Jurkat cells with hnRNP A1 (4H7) Monoclonal Antibody (bsm-54024R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).