bsm-54040R [Primary Antibody]
MMP9 Recombinant Antibody
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Host: Rabbit

Target Protein: MMP9

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4318

Swiss Prot: P14780

Source: A synthesized peptide derived from human MMP9

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 1mg/ml

Applications: WB(1:300-5000)
IHC-P(1:20-100)
IHC-F(1:200-400)
IF()

Predicted Molecular Weight: 100


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wenjun He. et al. Molecular Mechanism of Naringenin Against High-Glucose-Induced Vascular Smooth Muscle Cells Proliferation and Migration Based on Network Pharmacology and Transcriptomic Analyses.. FRONT PHARMACOL. 2022 Jun;13:862709-862709Read more>>
  • Ding Lingli. et al. Ginsenoside compound-K attenuates OVX-induced osteoporosis via the suppression of RANKL-induced osteoclastogenesis and oxidative stress. NAT PRODUCT BIOPROSP. 2023 Dec;13(1):1-12Read more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with MMP9 monoclonal antibody, unconjugated (bsm-54040R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


U2OS (H) cells were treated with or without TPA (200nM,48h) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with MMP9 monoclonal antibody, unconjugated (bsm-54040R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


MDA-MB-231 (H) cells were treated with or without TPA (200nM,24h) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with MMP9 monoclonal antibody, unconjugated (bsm-54040R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.