bsm-54040R
[Primary Antibody]
MMP9 (7D6) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MMP9
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4318
Swiss Prot: P14780
Source: C terminal human MMP9
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.
Background:
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 100
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wenjun He. et al. Molecular Mechanism of Naringenin Against High-Glucose-Induced Vascular Smooth Muscle Cells Proliferation and Migration Based on Network Pharmacology and Transcriptomic Analyses.. FRONT PHARMACOL. 2022 Jun;13:862709-862709Read more>>
- Ding Lingli. et al. Ginsenoside compound-K attenuates OVX-induced osteoporosis via the suppression of RANKL-induced osteoclastogenesis and oxidative stress. NAT PRODUCT BIOPROSP. 2023 Dec;13(1):1-12Read more>>
VALIDATION IMAGES
Lane 1: Rat Spleen; Probed with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Placenta tissue incubated with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, DAB staining.
Flow cytometric analysis of A431 cells with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Rat Spleen tissue lysates; Lane 2: Rat Lung tissue lysates probed with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Normal human U-2 OS cell lysates; Lane 2: U-2 OS treated with 200nM TPA for 48 hours lysates; Lane 3: Normal human MDA-MB-231 cell lysates; Lane 4: MDA-MB-231 serum starved overnight, then treated with 200nM TPA for 24 hours probed with MMP9 Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.