DATASHEET
Host:
Rabbit
Target Protein:
Bag3
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
9531
Swiss Prot:
O95817
Source:
N terminal human Bag3
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.
Background:
The Bag family of proteins are characterized by the presence of a 45 amino acid Bag domain through which they bind with high affinity to the ATPase domain of HSP 70, thereby negatively regulating HSP 70 chaperone activity. Bag-3 (Bcl-2-associated athanogene 3), also known as BIS or CAIR-1, is a 575 amino acid protein that contains one C-terminal Bag domain and two N-terminal WW domains. Like other members of the Bag family, Bag-3 functions to inhibit the chaperone activity of HSP 70, specifically by promoting the release of HSP 70-bound substrates. Additionally, Bag-3 exhibits anti-apoptotic activity via cell cycle control, suggesting a possible role for Bag-3 in tumor progression. The gene encoding Bag-3 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome.
VALIDATION IMAGES
Lane 1: Hela Cells; Lane 2: MCF-7 Cells; Probed with Bag3 (8D8) Monoclonal Antibody (bsm-54042R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with Bag3 (8D8) Monoclonal Antibody (bsm-54042R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.