support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Rabbit
Target Protein: GRIN2A
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 2903
Swiss Prot: Q12879
Source: A synthesized peptide derived from human GluN2A
Purification: Purified by Protein A.
Storage Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: Lot dependent
Applications:
WB(WB(1:200-1000))
IHC-P(IHC-P(1:50-200))
IHC-F(IHC-F(1:50-200))
IF(IHC-P)(IF(IHC-P)(1:50-200))
Predicted Molecular Weight: 163
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRIN2A Monoclonal Antibody, Unconjugated(bsm-54048R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human seahorse; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRIN2A Monoclonal Antibody, Unconjugated(bsm-54048R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with GRIN2A monoclonal antibody, unconjugated (bsm-54048R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRIN2A Monoclonal Antibody, Unconjugated (bsm-54048R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.