VALIDATION IMAGES
Lane 1: 293T Cells; Probed with Sin3A (3A6) Monoclonal Antibody (bsm-54058R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Sin3A (3A6) Monoclonal Antibody (bsm-54058R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Sin3A (3A6) Monoclonal Antibody (bsm-54058R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Sin3A (3A6) Monoclonal Antibody (bsm-54058R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Sin3A (3A6) Monoclonal Antibody (bsm-54058R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of MCF-7 cells with Sin3A (3A6) Monoclonal Antibody (bsm-54058R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).