VALIDATION IMAGES
Lane 1: SH-SY5Y Cells; Probed with PLGF (3F11) Monoclonal Antibody (bsm-54066R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with PLGF (3F11) Monoclonal Antibody (bsm-54066R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PLGF (3F11) Monoclonal Antibody (bsm-54066R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with PLGF (3F11) Monoclonal Antibody (bsm-54066R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with PLGF (3F11) Monoclonal Antibody (bsm-54066R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with PLGF (3F11) Monoclonal Antibody (bsm-54066R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Orchis tissue incubated with PLGF (3F11) Monoclonal Antibody (bsm-54066R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.