bsm-54069R [Primary Antibody]
DNA Polymerase beta (1C7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DNA Polymerase beta

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5423

Swiss Prot: P06746

Source: Human DNA Polymerase beta aa 300 to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: PC-12 Cells; Lane 2: NIH-3T3 Cells; Lane 3: A431 Cells; Probed with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Uterus tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Zebrafish Tissue; Probed with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:200 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.