bsm-54075R [Primary Antibody]
Fascin (12A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Fascin

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6624

Swiss Prot: Q16658

Source: Full length protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 54


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SH-SY5Y Cells; Lane 2: Human Brain; Lane 3: Rat Spleen; Lane 4: Mouse Testis; Lane 5: Mouse Brain; Lane 6: Mouse Spleen; Probed with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).