bsm-54077R [Primary Antibody]
Dynamin 2 (11G1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Dynamin 2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1785

Swiss Prot: P50570

Source: C terminal human Dynamin 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis . Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 98


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: PC-12 Cells; Lane 2: Mouse Thymus; Lane 3: Hela Cells; Probed with Dynamin 2 (11G1) Monoclonal Antibody (bsm-54077R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Dynamin 2 (11G1) Monoclonal Antibody (bsm-54077R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Dynamin 2 (11G1) Monoclonal Antibody (bsm-54077R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Dynamin 2 (11G1) Monoclonal Antibody (bsm-54077R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with Dynamin 2 (11G1) Monoclonal Antibody (bsm-54077R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Dynamin 2 (11G1) Monoclonal Antibody (bsm-54077R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Dynamin 2 (11G1) Monoclonal Antibody (bsm-54077R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Fallopian tissue incubated with Dynamin 2 (11G1) Monoclonal Antibody (bsm-54077R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with Dynamin 2 (11G1) Monoclonal Antibody (bsm-54077R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).