DATASHEET
Host:
Rabbit
Target Protein:
NFKBIE
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
4794
Swiss Prot:
O00221
Source:
Full length protein
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
VALIDATION IMAGES
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with NFKBIE (7A4) Monoclonal Antibody (bsm-54094R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: THP-1 Cells; Lane 2: Jurkat Cells; Lane 3: Hela Cells; Probed with NFKBIE (7A4) Monoclonal Antibody (bsm-54094R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.