bsm-54094R [Primary Antibody]
IKB epsilon Recombinant Antibody
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Host: Rabbit

Target Protein: IKB epsilon

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4794

Swiss Prot: O00221

Source: Recombinant human IKB epsilon protein

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:500-1000)
IHC-F(1:50-200)
IF(1:50-200)
IHC-P(1:50-200)
ICC/IF(1:50-200)
IP(1:20-50)

Predicted Molecular Weight: 45 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human U937 cell lysates; Lane 2: Human Raji cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human Hela cell lysates; Lane 6: Human HepG2 cell lysates probed with NFKBIE Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.