DATASHEET
Host:
Rabbit
Target Protein:
Skp1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
6500
Swiss Prot:
P63208
Source:
Full length protein
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Background:
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
VALIDATION IMAGES
Lane 1: PC-12 Cells; Probed with Skp1 (1C14) Monoclonal Antibody (bsm-54095R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Skp1 (1C14) Monoclonal Antibody (bsm-54095R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Skp1 (1C14) Monoclonal Antibody (bsm-54095R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Skp1 (1C14) Monoclonal Antibody (bsm-54095R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).