bsm-54104R [Primary Antibody]
phospho-MLKL (Ser345) Recombinant Antibody
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Host: Rabbit

Target Protein: phospho-MLKL (Ser345)

Modification Site: Ser345

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 74568

Swiss Prot: Q9D2Y4

Source: Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:500-2000)
ICC/IF(1:50-200)

Predicted Molecular Weight: 54


Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ying Tu. et al. Developmental exposure to chlorpyrifos causes neuroinflammation via necroptosis in mouse hippocampus and human microglial cell line. ENVIRON POLLUT. 2022 Dec;314:120217Read more>>
  • Liangyu Tan. et al. Regulation of RIP1-Mediated necroptosis via necrostatin-1 in periodontitis. J PERIODONTAL RES. 2023 JuRead more>>
  • Yu-qiong He. et al. Ursodeoxycholic acid alleviates sepsis-induced lung injury by blocking PANoptosis via STING pathway. INT IMMUNOPHARMACOL. 2023 Dec;125:111161Read more>>
  • Shasha Chen. et al. Quercetin alleviates zearalenone-induced apoptosis and necroptosis of porcine renal epithelial cells by inhibiting CaSR/CaMKII signaling pathway. FOOD CHEM TOXICOL. 2023 Nov;:114184Read more>>
  • Dan Zhao. et al. Copper exposure induces inflammation and PANoptosis through the TLR4/NF-B signaling pathway, leading to testicular damage and impaired spermatogenesis in Wilson disease. CHEM-BIOL INTERACT. 2024 Jun;396:11106Read more>>
  • Haoyu Zhang. et al. Timing, initiation and function: An in-depth exploration of the interaction network among neutrophil extracellular traps related genes in acute pancreatitis. INT IMMUNOPHARMACOL. 2024 Nov;141:112923Read more>>
VALIDATION IMAGES

L-929 (M) cells were treated with or without Z-VAD (20 μM) for 30 min, mTNF-α (20 ng/ml) for 4 h, SM-164 (100 nM) for 4 h, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-MLKL (Ser345) monoclonal antibody, unconjugated (bsm-54104R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.