bsm-54104R [Primary Antibody]
MLKL (Ser345) (7G4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MLKL Ser345

Modification Site: Ser345

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 74568

Swiss Prot: Q9D2Y4

Source: Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 54


Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ying Tu. et al. Developmental exposure to chlorpyrifos causes neuroinflammation via necroptosis in mouse hippocampus and human microglial cell line. ENVIRON POLLUT. 2022 Dec;314:120217Read more>>
  • Liangyu Tan. et al. Regulation of RIP1-Mediated necroptosis via necrostatin-1 in periodontitis. J PERIODONTAL RES. 2023 JuRead more>>
  • Yu-qiong He. et al. Ursodeoxycholic acid alleviates sepsis-induced lung injury by blocking PANoptosis via STING pathway. INT IMMUNOPHARMACOL. 2023 Dec;125:111161Read more>>
  • Shasha Chen. et al. Quercetin alleviates zearalenone-induced apoptosis and necroptosis of porcine renal epithelial cells by inhibiting CaSR/CaMKII signaling pathway. FOOD CHEM TOXICOL. 2023 Nov;:114184Read more>>
VALIDATION IMAGES

Lane 1: Mouse Spleen; Lane 2: Mouse Lung; Lane 3: Mouse Thymus; Probed with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.