bsm-54106R [Primary Antibody]
GABARAP (3A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GABARAP

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 11337

Swiss Prot: O95166

Source: Human GABARAP aa 1-100

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 14


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Kidney; Lane 2: Rat Liver; Probed with GABARAP (3A1) Monoclonal Antibody (bsm-54106R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with GABARAP (3A1) Monoclonal Antibody (bsm-54106R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with GABARAP (3A1) Monoclonal Antibody (bsm-54106R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with GABARAP (3A1) Monoclonal Antibody (bsm-54106R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with GABARAP (3A1) Monoclonal Antibody (bsm-54106R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).