DATASHEET
Host:
Rabbit
Target Protein:
ENO1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
2023
Swiss Prot:
P06733
Source:
Synthetic peptide within N-terminal human ENO1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Background:
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
VALIDATION IMAGES
Flow cytometric analysis of MCF-7 cells with ENO1 (4B3) Monoclonal Antibody (bsm-54109R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Rat Brain; Lane 2: NIH-3T3; Lane 3: Hela Cells; Lane 4: HepG2 Cells; Probed with ENO1 (4B3) Monoclonal Antibody (bsm-54109R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Zebrafish tissue lysates; Probed with ENO1 (4B3) Monoclonal Antibody (bsm-54109R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.