bsm-54109R [Primary Antibody]
ENO1 (4B3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ENO1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2023

Swiss Prot: P06733

Source: Synthetic peptide within N-terminal human ENO1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of MCF-7 cells with ENO1 (4B3) Monoclonal Antibody (bsm-54109R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Rat Brain; Lane 2: NIH-3T3; Lane 3: Hela Cells; Lane 4: HepG2 Cells; Probed with ENO1 (4B3) Monoclonal Antibody (bsm-54109R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Zebrafish tissue lysates; Probed with ENO1 (4B3) Monoclonal Antibody (bsm-54109R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.