bsm-54110R [Primary Antibody]
Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PDHB

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5162

Swiss Prot: P11177

Source: Recombinant protein within C-terminal human Pyruvate Dehydrogenase E1 beta subunit

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency). PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HepG2 Cells; Lane 2: Hela Cells; Probed with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Kidney; Lane 2: PC-12 Cells; Probed with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Hippocampus tissue incubated with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).