VALIDATION IMAGES
Lane 1: HepG2 Cells; Lane 2: Hela Cells; Probed with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Kidney; Lane 2: PC-12 Cells; Probed with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Hippocampus tissue incubated with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Hela cells with Pyruvate Dehydrogenase E1 beta subunit (10A1) Monoclonal Antibody (bsm-54110R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).