bsm-54111R [Primary Antibody]
SMYD3 (4C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SMYD3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 64754

Swiss Prot: Q9H7B4

Source: Recombinant protein within human SMYD3 aa 200-350

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain. Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Also methylates 'Lys-5' of histone H4. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Rat Kidney; Lane 2: PC-12; Lane 3: Hela Cells; Lane 4: Mouse Spleen; Lane 5: NIH-3T3 Cells; Lane 5: Rat Spleen; Probed with SMYD3 (4C9) Monoclonal Antibody (bsm-54111R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with SMYD3 (4C9) Monoclonal Antibody (bsm-54111R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with SMYD3 (4C9) Monoclonal Antibody (bsm-54111R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with SMYD3 (4C9) Monoclonal Antibody (bsm-54111R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated SMYD3 (4C9) Monoclonal Antibody (bsm-54111R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated SMYD3 (4C9) Monoclonal Antibody (bsm-54111R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated SMYD3 (4C9) Monoclonal Antibody (bsm-54111R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Stomach tissue incubated SMYD3 (4C9) Monoclonal Antibody (bsm-54111R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with SMYD3 (4C9) Monoclonal Antibody (bsm-54111R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).