bsm-54114R [Primary Antibody]
WDR5 (8G9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: WDR5

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 11091

Swiss Prot: P61964

Source: Synthetic peptide within human WDR5 aa 300 to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela Cells; Lane 2: A549 Cells; Lane 3: NIH-3T3 Cells; Lane 4: PC-12 Cells; Probed with WDR5 (8G9) Monoclonal Antibody (bsm-54114R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with WDR5 (8G9) Monoclonal Antibody (bsm-54114R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with WDR5 (8G9) Monoclonal Antibody (bsm-54114R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).