bsm-54117R [Primary Antibody]
Nogo-B (7A12) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Nogo-B

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 57142

Swiss Prot: Q9NQC3

Source: Recombinant protein within human Nogo aa 1-200

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Reticulon-4 is a developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. It regulates neurite fasciculation, branching and extension in the developing nervous system. Reticulon-4 is also Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Isoform 2 of the Reticulon-4 protein, also known as Nogo-B or Reticulon-4B, reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 (Nogo-B) and isoform 3 (Nogo-C) inhibit BACE1 activity and amyloid precursor protein processing. Nogo-B is mainly localized primarily in the endoplasmic reticulum (ER), where it participates in the maintenance of ER tubular shape and functions. Nogo-B is expressed in most tissues and is associated with diverse processes such as vascular remodeling, liver regeneration, liver cirrhosis, pulmonary hypertension, and inflammatory bowel disease.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: Hu: 55/42; Mo: 50/38


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Skeleal Muscle; Lane 2: Hela Cells; Probed with Nogo-B (7A12) Monoclonal Antibody (bsm-54117R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Nogo-B (7A12) Monoclonal Antibody (bsm-54117R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Nogo-B (7A12) Monoclonal Antibody (bsm-54117R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Nogo-B (7A12) Monoclonal Antibody (bsm-54117R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated Nogo-B (7A12) Monoclonal Antibody (bsm-54117R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated Nogo-B (7A12) Monoclonal Antibody (bsm-54117R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated Nogo-B (7A12) Monoclonal Antibody (bsm-54117R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated Nogo-B (7A12) Monoclonal Antibody (bsm-54117R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with Nogo-B (7A12) Monoclonal Antibody (bsm-54117R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).