VALIDATION IMAGES
Lane 1: Hela Cells; Lane 2: PC-12 Cells; Lane 3: A431 Cells; Probed with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of A431 cells with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).