bsm-54120R [Primary Antibody]
PFKFB3 (1G2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PFKFB3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5209

Swiss Prot: Q16875

Source: Recombinant protein within human PFKFB3 aa 300 to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 60


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela Cells; Lane 2: PC-12 Cells; Lane 3: A431 Cells; Probed with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of A431 cells with PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).