bsm-54125R [Primary Antibody]
RANTES Recombinant Antibody
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Host: Rabbit

Target Protein: RANTES

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 6352

Swiss Prot: P13501

Source: Recombinant protein.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.

Size: 100µL

Concentration: 1ug/ul

Predicted Molecular Weight: 10


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CCL5 (6A1) Monoclonal Antibody (bsm-54125R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with CCL5 (6A1) Monoclonal Antibody (bsm-54125R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


THP-1 (H) cells were treated with or without PMA (100ng/ml) for 48 h, and LPS (1ug/ml) for 8 h, and Brefeldin A (600ng/ml) for 3 h, 25 μg total protein per lane of cell lysates (see on figure) probed with RANTES monoclonal antibody, unconjugated (bsm-54125R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with CCL5 (6A1) Monoclonal Antibody (bsm-54125R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.