bsm-54126R [Primary Antibody]
DDAH1 (2G4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DDAH1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 23576

Swiss Prot: O94760

Source: Recombinant protein within human DDAH1 aa 100-285

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. DDAH1 hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 31


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human Kidney; Lane 2: Human Liver; Probed with DDAH1 (2G4) Monoclonal Antibody (bsm-54126R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with DDAH1 (2G4) Monoclonal Antibody (bsm-54126R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with DDAH1 (2G4) Monoclonal Antibody (bsm-54126R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.