DATASHEET
Host:
Rabbit
Target Protein:
DDAH1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
23576
Swiss Prot:
O94760
Source:
Recombinant protein within human DDAH1 aa 100-285
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Background:
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. DDAH1 hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS.
VALIDATION IMAGES
Lane 1: Human Kidney; Lane 2: Human Liver; Probed with DDAH1 (2G4) Monoclonal Antibody (bsm-54126R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with DDAH1 (2G4) Monoclonal Antibody (bsm-54126R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with DDAH1 (2G4) Monoclonal Antibody (bsm-54126R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.