bsm-54128R [Primary Antibody]
UBA2 (4F12) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: UBA2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10054

Swiss Prot: Q9UBT2

Source: Recombinant protein within human UBA2 aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

UBA2 (aka SAE2) functions to induce posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, which regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 71


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A549 Cells; Lane 2: PC-12 Cells; Probed with UBA2 (4F12) Monoclonal Antibody (bsm-54128R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.