bsm-54131R [Primary Antibody]
DNA Ligase IV (2D9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LIG4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3981

Swiss Prot: P49917

Source: Recombinant protein within human DNA Ligase IV aa 500-700

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 104


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HepG2 Cells; Lane 2: Hela Cells; Lane 3: Jurkat Cells; Probed with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Human MCF-7 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HT-1080 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human Ramos cell lysates; Lane 6: Human HepG2 cell lysates probed with DNA Ligase IV Monoclonal Antibody, Unconjugated (bsm-54131R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.