VALIDATION IMAGES
Lane 1: HepG2 Cells; Lane 2: Hela Cells; Lane 3: Jurkat Cells; Probed with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HT-1080 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human Ramos cell lysates; Lane 6: Human HepG2 cell lysates probed with DNA Ligase IV Monoclonal Antibody, Unconjugated (bsm-54131R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.