bsm-54133R [Primary Antibody]
RAPGEF3 (3D1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RAPGEF3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10411

Swiss Prot: O95398

Source: Recombinant protein within human Epac1 aa 700-900

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 104


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with RAPGEF3 (3D1) Monoclonal Antibody (bsm-54133R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with RAPGEF3 (3D1) Monoclonal Antibody (bsm-54133R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with RAPGEF3 (3D1) Monoclonal Antibody (bsm-54133R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Mouse Kidney; Probed with RAPGEF3 (3D1) Monoclonal Antibody (bsm-54133R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.