VALIDATION IMAGES
Lane 1: PC-12 Cells; Lane 2: Jurkat Cells; Lane 3: NIH-3T3 Cells; Probed with JAK1 (2B12) Monoclonal Antibody (bsm-54138R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with JAK1 (2B12) Monoclonal Antibody (bsm-54138R) at 1:50, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of SW480 cells with JAK1 (2B12) Monoclonal Antibody (bsm-54138R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (JAK1 (2B12)) Monoclonal Antibody, Unconjugated (bsm-54138R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with JAK1 (2B12) Monoclonal Antibody (bsm-54138R) at 1:50, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (JAK1 (2B12)) Monoclonal Antibody, Unconjugated (bsm-54138R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.