VALIDATION IMAGES
IF(ICC) staining with Trefoil factor 1 (4B1) Monoclonal Antibody (bsm-54139R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Trefoil factor 1 (4B1) Monoclonal Antibody (bsm-54139R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Trefoil factor 1 (4B1) Monoclonal Antibody (bsm-54139R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with Trefoil factor 1 (4B1) Monoclonal Antibody (bsm-54139R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Trefoil factor 1 (4B1) Monoclonal Antibody (bsm-54139R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of MCF-7 cells with Trefoil factor 1 (4B1) Monoclonal Antibody (bsm-54139R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).