bsm-54140R [Primary Antibody]
NRF1 (6G5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NRF1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4899

Swiss Prot: Q16656

Source: Recombinant protein within human NRF1 aa 300-450

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of 293T cells with NRF1 (6G5) Monoclonal Antibody (bsm-54140R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with NRF1 (6G5) Monoclonal Antibody (bsm-54140R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Thyroid tissue incubated with NRF1 (6G5) Monoclonal Antibody (bsm-54140R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with NRF1 (6G5) Monoclonal Antibody (bsm-54140R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with NRF1 (6G5) Monoclonal Antibody (bsm-54140R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with NRF1 (6G5) Monoclonal Antibody (bsm-54140R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with NRF1 (6G5) Monoclonal Antibody (bsm-54140R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with NRF1 (6G5) Monoclonal Antibody (bsm-54140R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: Hela Cells; Lane 2: Mouse Heart; Probed with NRF1 (6G5) Monoclonal Antibody (bsm-54140R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.