bsm-54140R [Primary Antibody]
NRF1 Recombinant Antibody
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Host: Rabbit

Target Protein: NRF1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4899

Swiss Prot: Q16656

Source: Recombinant protein within human NRF1 aa 300-450

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Size: 100µL

Concentration: Lot Dependent

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NRF1 Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with NRF1 monoclonal antibody, unconjugated (bsm-54140R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.