bsm-54141R [Primary Antibody]
GCLM (4G5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GCLM

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2730

Swiss Prot: P48507

Source: Synthetic peptide within human GCLM aa 200-250

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 31


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of Hela cells with GCLM (4G5) Monoclonal Antibody (bsm-54141R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with GCLM (4G5) Monoclonal Antibody (bsm-54141R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with GCLM (4G5) Monoclonal Antibody (bsm-54141R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with GCLM (4G5) Monoclonal Antibody (bsm-54141R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with GCLM (4G5) Monoclonal Antibody (bsm-54141R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with GCLM (4G5) Monoclonal Antibody (bsm-54141R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with GCLM (4G5) Monoclonal Antibody (bsm-54141R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: A431 Cells; Lane 2: PC-12 Cells; Lane 3: NIH-3T3 Cells; Probed with GCLM (4G5) Monoclonal Antibody (bsm-54141R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.