VALIDATION IMAGES
Lane 1: Human Fetal Brain; Lane 2: HepG2 Cells; Lane 3: 293 Cells; Probed with FTO (12A1) Monoclonal Antibody (bsm-54143R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with FTO (12A1) Monoclonal Antibody (bsm-54143R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with FTO (12A1) Monoclonal Antibody (bsm-54143R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Uterus tissue incubated with FTO (12A1) Monoclonal Antibody (bsm-54143R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with FTO (12A1) Monoclonal Antibody (bsm-54143R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with FTO (12A1) Monoclonal Antibody (bsm-54143R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.