bsm-54146R [Primary Antibody]
Aldolase A (2C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ALDOA

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 226

Swiss Prot: P04075

Source: Recombinant protein within human Aldolase aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. In addition, may also function as a scaffolding protein. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse
Rat
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100 in SK-BR-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: Zebrafish Tissue; Probed with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:200 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: A549 Cells; Lane 2: Hela Cells; Lane 3: Mouse Liver; Lane 4: Rat Liver; Lane 5: Rat Spleen; Probed with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.