bsm-54150R
[Primary Antibody]
Rad51 (11D1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Rad51
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5888
Swiss Prot: Q06609
Source: Recombinant protein within human Rad51 aa 1-150
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 37
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Hela Cells; Lane 2: Human Kidney; Lane 3: Mouse Testis; Probed with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 in PANC-1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Testis tissue incubated with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Jurkat, 2: NIH/3T3, 3: C6 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 37 kDa Observed MW: 37 kDa
Flow cytometric analysis of Jurkat cells with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Tissue: Human testis Section type: Formalin-fixed & Paraffinembedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54150R
Tissue: Rat testis Section type: Formalin-fixed & Paraffinembedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-54150R
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates probed with Rad51 Monoclonal Antibody, Unconjugated (bsm-54150R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.