bsm-54151R

DATASHEET

Host: Rabbit

Target Protein: H2AFX

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3014

Swiss Prot: P16104

Source: Synthetic peptide within human Histone H2A.X aa 1-50

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)
IHC-P(1:200-400)

Predicted Molecular Weight: 14

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.