bsm-54157R [Primary Antibody]
ACTN4 (6A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ACTN4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 81

Swiss Prot: O43707

Source: Recombinant protein within human alpha Actinin 4 aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 205


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela Cells; Lane 2: PC-12 Cells; Lane 3: NIH-3T3 Cells; Lane 4: Rat Liver; Lane 5: A431 Cells; Lane 6: HepG2 Cells; Probed with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of A549 cells with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).