bsm-54160R [Primary Antibody]
HPRT1 (6C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HPRT1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3251

Swiss Prot: P00492

Source: Recombinant protein within human HPRT aa 1-200

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this protein result in Lesch-Nyhan syndrome or gout.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 25


Cross Reactive Species: Human
Mouse
Rat
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Hela Cells; Lane 2: A431 Cells; Lane 3: MCF-7 Cells; Lane 4: 293 Cells; Lane 5: Rat Kidney; Lane 6: Rat Brain; Lane 7: Mouse Testis; Lane 8: Mouse Colon; Probed with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Zebrafish Tissue; Probed with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.