bsm-54166R [Primary Antibody]
USP13 (1G5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: USP13

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8975

Swiss Prot: Q92995

Source: Recombinant protein within human USP13 aa 700-850

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 97


Cross Reactive Species: Human
Mouse
(WB(1:500-2000), IF(ICC)(1:50-200), FCM(1:50-100))

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Testis; Probed with USP13 (1G5) Monoclonal Antibody (bsm-54166R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with USP13 (1G5) Monoclonal Antibody (bsm-54166R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with USP13 (1G5) Monoclonal Antibody (bsm-54166R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with USP13 (1G5) Monoclonal Antibody (bsm-54166R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Flow cytometric analysis of SH-SY5Y cells with USP13 (1G5) Monoclonal Antibody (bsm-54166R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).