bsm-54170R
[Primary Antibody]
PSMA1 (10A2) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PSMA1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5682
Swiss Prot: P25786
Source: Recombinant protein within human PSMA1 aa 1-200
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 30
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: HepG2 Cells; Lane 4: 293 Cells; Probed with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: PC-12 Cells; Lane 2: Mouse Spleen; Lane 3: Rat Spleen; Probed with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of HepG2 cells with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA1 (10A2) Monoclonal Antibody, Unconjugated (bsm-54170R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse Cerebellum tissue lysates; Lane 4: Mouse Liver tissue lysates; Lane 5: Rat Spleen tissue lysates; Lane 6: Rat Testis tissue lysates; Lane 7: Rat Cerebellum tissue lysates; Lane 8: Rat Pancreas tissue lysates; Lane 9: Rat Liver tissue lysates; Lane 10: Human Hela cell lysates; Lane 11: Human Jurkat cell lysates; Lane 12: Human MCF-7 cell lysates; Lane 13: Human HepG2 cell lysates probed with PSMA1 Polyclonal Antibody, Unconjugated (bsm-54170R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.