bsm-54174R

DATASHEET

Host: Rabbit

Target Protein: SAMHD1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 25939

Swiss Prot: Q9Y3Z3

Source: Synthetic peptide within human SAMHD1 aa 600 to the C-terminus

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

SAMHD1 may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. SAMHD1 is a cellular enzyme, responsible for blocking replication of HIV in dendritic cells, macrophages and monocytes. It is an enzyme that exhibits phosphohydrolase activity, converting deoxynucleoside triphosphates (dNTPs) to inorganic phosphate (iPPP) and a 2'-deoxynucleoside (i.e. deoxynucleosides without a phosphate group). In doing so, SAMHD1 depletes the pool of dNTPs available to a reverse transcriptase for viral cDNA synthesis and thus prevents viral replication. SAMHD1 has also shown nuclease activity. Although a ribonuclease activity was described to be required for HIV-1 restriction, recent data confirmed that SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.

Size: 100ul, 20uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 72

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.