bsm-54178R [Primary Antibody]
Histone H3 (acetyl K14) Recombinant Antibody
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Host: Rabbit

Target Protein: Histone H3 (acetyl K14)

Modification Site: acetyl K14

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 8350

Swiss Prot: P68431

Source: Synthetic peptide of human Histone H3 (acetyl K14) coupled with KLH

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)
ICC/IF(1:50-100)

Predicted Molecular Weight: 15-Nov


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 (acetyl K14) Monoclonal Antibody, Unconjugated (bsm-54178R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 (acetyl K14) Monoclonal Antibody, Unconjugated (bsm-54178R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 (acetyl K14) Monoclonal Antibody, Unconjugated (bsm-54178R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 (acetyl K14) Monoclonal Antibody, Unconjugated (bsm-54178R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 (acetyl K14) Monoclonal Antibody, Unconjugated (bsm-54178R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 (acetyl K14) Monoclonal Antibody, Unconjugated (bsm-54178R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


C2C12 (M) treated with Trichostatin A (TSA) (1μM) for 18 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Histone H3 (acetyl K14) monoclonal antibody, unconjugated (bsm-54178R) at 1:800 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.