bsm-54178R [Primary Antibody]
Histone H3 (acetyl K14) (1C6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Histone H3 (acetyl K14)

Modification Site: acetyl K14

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8350

Swiss Prot: P68431

Source: Synthetic peptide of human Histone H3 (acetyl K14) coupled with KLH

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 15-Nov


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Rat Liver; Lane 2: Mouse Lung; Probed with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Jurkat Cells; Probed with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:200 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:200 in MCF-7cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:200 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.