bsm-54180R [Primary Antibody]
SDHB (4C7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SDHB

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6390

Swiss Prot: P21912

Source: Recombinant protein within human SDHB aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of Jurkat cells with SDHB (4C7) Monoclonal Antibody (bsm-54180R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Esophagus tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Human Liver; Lane 2: Rat Spleen; Lane 3: Rat Liver; Lane 4: Mouse Spleen; lane 5: HepG2 Cells; Probed with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.