DATASHEET
Host:
Rabbit
Target Protein:
Involucrin
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
3713
Swiss Prot:
P07476
Source:
Recombinant protein within human Involucrin aa 50-250
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Background:
Involucrin is a precursor protein of the keratinocyte cornified envelope, which is formed beneath the inner surface of the cell membrane during terminal differentiation. During keratinocyte terminal differentiation, glutamine residues of involucrin become covalently cross-linked to other envelope precursors via covalent epsilon-(gamma-glutamyl) lysine bonds. Moreover, its large size allows involucrin to cross-link molecules that are separated by substantial distances in the cornified envelope. These properties allow a single involucrin molecule to form multiple cross-links, in multiple spatial planes, with other envelope precursors.
VALIDATION IMAGES
Flow cytometric analysis of A431 cells with Involucrin (7C8) Monoclonal Antibody (bsm-54182R) 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with Involucrin (7C8) Monoclonal Antibody (bsm-54182R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Skin Tissue; Probed with Involucrin (7C8) Monoclonal Antibody (bsm-54182R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.