bsm-54184R [Primary Antibody]
Drosha (5C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Drosha

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 29102

Swiss Prot: Q9NRR4

Source: Recombinant protein within human Drosha aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 159


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela Cells; Lane 2: SiHa Cells; Probed with Drosha (5C9) Monoclonal Antibody (bsm-54184R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Drosha (5C9) Monoclonal Antibody (bsm-54184R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Drosha (5C9) Monoclonal Antibody (bsm-54184R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Drosha (5C9) Monoclonal Antibody (bsm-54184R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Drosha (5C9) Monoclonal Antibody (bsm-54184R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Drosha (5C9) Monoclonal Antibody (bsm-54184R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Jurkat cells with Drosha (5C9) Monoclonal Antibody (bsm-54184R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: K562 Cells; Probed with Drosha (5C9) Monoclonal Antibody (bsm-54184R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.