bsm-54185R [Primary Antibody]
Transglutaminase 2 (2C2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Transglutaminase 2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7052

Swiss Prot: P21980

Source: Synthetic peptide within human Transglutaminase 2 aa 550-600

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 77


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Transglutaminase 2 (2C2) Monoclonal Antibody (bsm-54185R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: HUVEC Cells; Probed with Transglutaminase 2 (2C2) Monoclonal Antibody (bsm-54185R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Placenta; Lane 2: Mouse Lung; Probed with Transglutaminase 2 (2C2) Monoclonal Antibody (bsm-54185R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.